The SpectraTest® ABS1. Protein concentrations were calculated using polynomial 2nd analysis from the protein standards. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. The multi-channel design truly mimics a. For very concentrated samples, use a minimum0200-6262. Not all manufacturers microplates are the same with regard to design, mate- rials, or conÞguration. 16 $ $ 36,002 . 5 µL - Detector: CCD array, 1024 element - Nguồn sáng : đèn Xenon - Nồng độ. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]®Plus384addstheabilitytoread cuvettes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Simplify and expedite experiments with SoftMax Pro Software’s. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The 200 μL mixture was added into a 96-well plate, and the absorbance was measured at 517 nm by enzyme-labeled instrument (SpectraMax 190, Shanghai, China). The patented multichannel optical design of the SpectraMax® 190 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate. The data are shown in optical density (O. (SpectraMax ® 190, Molecular Devices). 2. * dsDNA sensitivity specified for the following instruments: SpectraMax® 190 Readers, SpectraMax® Plus384 Readers,. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 0 out of 5 $ 2,499 $ 1,999. SOFTmax PRO. According to the manufacturer, the sensitivity of this assay is 5 ng/ml and has less than 1% cross. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. Exceptional performanceThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. This instrument is designed to answer the strict requirements of. Bioz Stars score: 98/100, based on 1 PubMed citations. , Tokyo, Japan) was added to each well every 24 h. A layer of growth factor-reduced Matrigel was made for 3D cultures . 303 unable to cal dark current. ₹ 1,80,000 Get Latest Price. The multi-channel design truly mimics a dual-beam spectrophotometer. The VersaMax and SpectraMax 190 read 96-well microplates. We assessed the expression of pro- and anti-inflammatory genes expressed by macrophages, including. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. . The SpectraMax 190 has a multichannel design for 96-well Molecular Devices SpectraMax 190 Temperature range: 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices SpectraMax 190 can be used to quantify DNA and perform endpoint, kinetic, and spectral scanning analyses. 9000-0756. DNA provides the same sensitivity as a spectrophotometer. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Spectramax Plus 384 Plate Reader. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. The equation that allows one to calculate absorbance from % transmittance is. The cell viability was normalised to the control group. A. Plasma biomarker levels were measured by enzyme-linked immunosorbent assay (ELISA; R&D unless otherwise stated) using a SpectraMax 190 plate reader (SpectraMax 190 microplate reader, RRID : SCR_018932). The narrow bandwidth provides greater measurement accuracy and linearity over the widest assay range, including DNA. The SpectraMax 190 Microplate Reader from Molecular Devices is ideal for UV-Vis life science applications, especially DNA analysis. 17247 MOLECULAR DEVICES MICROPLATE SPECTROPHOTOMETER SPECTRAMAX 190. Optional tools for verifying the SpectraMax 190 reader performance include the SpectraTest ABS1 Absorbance Plate and IQ/OQ/PQ manual. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. Phone Number Login / Register. Photometric accuracy. Temperature control and shaking come as standard. Wavelength bandwidth ≤ 2. SpectraMax 190 and Spect FRET fluorescence, top- le-Mode Detection Fluorescence Sir and bottom-read, cell-based assay, lal injection in both 96- and 384-well lates, excellent sensitivity, Autorinse range, du microp 0+ pre-defined protocols; lac compatible based (visible 405-750 nm)The obtained mixture was reacted in a boiling water bath for 20 min, and then, cooled down immediately. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. When the user applies a set temperature of 37°C, the top is heated to 37. 00. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area. In vitro blood test. TECHNICAL NOTE. Soluble PA fractions (20 μL) were mixed with 100 μL of DMACA reagent on a 96-well plate. On LabX buyers can find. When connecting for the first time to either the SpectraMax L reader or StakMax system. Add to Cart Buy. It measures the optical density (OD) of samples in both 96- and 384-well microplates or in a cuvette at a selected wavelength. Fluorescence and fluorescence polarization. overnight at room. SpectraMax Gemini EM & FLEXstation (bottom read) 1. Our Price: $10,995. The clearance of DPPH was calculated by the. Tumor cells were plated onto 96-well plates (500 cells/well), and 10 µl of Premix WST-1 Cell Proliferation Assay System (Takara Bio Inc. 9 in. Italic and boldface type are used for emphasis. ®{"general":{"total":5,"offset":0,"limit":5,"data":[{"key":"label","path":"path","name":"Name","identifier":"Identifier","wavelength-ranges":"Wavelength ranges. Molecular Devices’ PathCheck® sensor technology. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The Solution Speaking about how OligoMaker uses the SpectraMax 190, Karl Ross-Petersen explains, "We use the SpectraMax 190 as an important step in testing the DNA/RNA. The VersaMax and SpectraMax 190 read 96-well microplates. Both. #3635 or Greiner cat. Ii SPECTRAmax 190 Microplate Spectrophotometer. D. Price: $2,200. Description. Do not use a dry plate. Customer Satisfaction is our number one goal All lab equipment in stock already tested and ships in 1-3 days• The TRIAX 180/190 models have four leveling feet and four foot pads. A = Log 10 (I 0 /I). 8 ng/mL. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. • Last day to purchase a service plan for SpectraMax 190 Microplate Reader is December 31, 2027. Italic and boldface type are used for emphasis. Molecular Devices SpectraMax 340PC384 Microplate Reader $ 4,000. The absorbance was read every 30 s for a period of 3 min using a SpectraMax 190 microplate reader. 1) Turn on the power switch of the SPECTRAmax 190 (located on the back panel). Add to Cart. A 5 mL of aqueous solution of VOSO 4 (0. . View our entire inventory of New Or Used Roadtrek RVs. The Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. Also for: Spectramax plus. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. After 1 h of incubation, the absorbance of each well was measured at A450 nm using a SpectraMax microplate reader (SpectraMax 190, Molecular Devices, USA). 0 nm. 验证检测 SpectraMax 190 的工作状态。 堆板机、机械臂一体化整合 SpectraMax 190 可以在几分钟内整合到 Molecular Devices 的 StakMax 堆板机上。为了实现更高程度的自动化，自动化供应商 合作伙伴已经把我们的酶标仪系统和所有主要合作伙伴的机械臂 进行了整合。 The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. SoftMax® Pro Software User Guide — 0112-0140 Rev. Minimize sample volume while retaining sensitivity with the SpectraDrop Micro-Volume Microplate. Wavelength Accuracy: < ± 2. . The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Id3 spectramax reader molecular microplate multi mode devices vwr std. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Add to Cart. To contact technical support by phone in the U. Optical absorbance and fluorescence were measured on a SpectraMax M5 Multi-Mode microplate reader (Molecular Devices, San Jose, CA). 00. For wavelengths in the UV range above 220 nm,. NO assayIMI detection was conducted using the Eurofins Imidacloprid ELISA kit (Eurofins Abraxis, Warminster, PA, USA) following the manufacturer’s instructions for extraction and quantification of IMI residues in leaf tissue and using a spectrophotometer (SpectraMax 190, Molecular Devices, San Jose, CA, USA). The final value was expressed as ng/g protein. spectramax i3x (Danaher Inc) Danaher Inc is a verified supplier. 190 – 1100 nm Wavelength accuracy : ±2 nm Stray light <0. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Spleen Cell. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- StakMax Handling Stacker Base Unit. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. Prepare your iD series instrument for shipment. Web Information The Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. SpectraMax iD3 and iD5 Register or Sign In. . The PathCheck sensor can extend the instrument linearity by a factor of three or more. ATP standard curves read on SpectraMax microplate readers. Bookings are free, but you will need to use iLab to book. Offer Ends In: 9 Days 19: 59: 40. The technology allows automatic adjustment of good absorbance to a tube equivalent path length of 1 cm, converted to 96 or 384 tubes. D) units. Molecular Devices Spectramax 190 Absorbance Microplate Reader. 00. Note: Due to. SpectraMax 190 Absorbance Microplate Reader with laptop and software Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 049 g, 0. 1. Free shipping. These models can no longer be repaired. Manufacturer: Molecular Devices; Model: SpectraMax 190 * Wavelength Selection Monochromator, tunable 1. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. mg −1 protein using the extinction coefficient of 39. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. 0 out of 5 $. After samples were incubated at room temperature for 4. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. Model: SpectraMax 250 Condition: Used. After this date, all service and support will be on a reasonable effort basis At this time, we are happy to help you upgrade to the SpectraMax® ABS or ABS Plus Microplate Readers which containThe solubility of compounds was measured using a SPECTRAMAX 190 (Molecular Devices, San Jose, CA, USA). The dichloromethane, methanol, and hexane extracts were eluted four times and the absorbance spectra of the samples was measured at a range of wavelengths from 200 to 750 nm. Fluorescent Polarization: Solid black plates, as above. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Molecular Devices SpectraMax 190 User Manual (55 pages) Microplate Spectrophotometers. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. The . Description 1. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Microplate Readers Promega GloMax Plate Reader Multi-Detection. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. Both microplate and cuvette ports can be qualified. Very small footprint suitable for tight space requirements. ScanLater Western Blot Detection Cartridge. STAKMAX. $500. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Double-click the unknown device with the yellow warning icon. Italic and boldface type are used for emphasis. The sensitivity of this assay was 250 pg/mL, and the intra. Near bottom. SPECTRAmax PLUS 384 measuring instruments pdf manual download. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Auction Details Bio + Laboratory Equipment Expo. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Wavelength range 190–1000 nm Wavelength selection Monochromator, tunable in 1. We have 1 Molecular Devices SpectraMax 190 manual available for free PDF download: User Manual . EZ-Cytox (10 µL) was added to each of the wells of the plate and incubated for 1 h. Italic and boldface type are used for emphasis. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. The key protein of Wnt/β-catenin signaling pathways expression Protein β-catenin, CD14, p-ERK and p-Jun N-terminal kinase (p-JNK) expression in Wnt/β-catenin signaling pathways of SSCs after treatment for each. Wavelength Accuracy: < ± 2. Multimode Reader Comparison Chart. 315 can't find zero order. Slopes from the two curves were. 5 - 2. Sale Price: $12,125. . Microplate Reader Shelf. SpectraMax 190 Absorbance Microplate Reader. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. The patented multichannel optical design of the SpectraMax® 190. Within the three main categories, microplate readers may differ in the maximum temperature enabled. Absorption was measured at 450 nm 24 h after premix added using SpectraMax 190 (Molecular Devices, LLC, Sunnyvale, CA, USA) to produce a cell. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Temperature control and shaking come as standard. com. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Tumor cells were plated onto 96-well plates (500 cells/well), and 10 µl of Premix WST-1 Cell Proliferation Assay System (Takara Bio Inc. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. The blood was centrifuged at 3000 rpm for 15. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Optical density was measured at 570 nm with an automatic microplate spectrophotometer (SpectraMax 190, Sunnyvale, USA). INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well a SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). Absorbance was acquired at 450 nM in the microplate reader SpectraMax 190. The SpectraMax 190 reader is the tool for DNA analysis. See SpectraMax® Microplate Reader User Manual Download Page. Location: California. ®System (PerkinElmer) On The SpectraMax ID5 Reader. The dual-scanning capability can also be used to determine excitation and emission settings for new fluorescent probes. Monochromator, tunable 1. 2. Colorimetric reaction was stopped by adding 2 NH2SO4. SpectraMax® Plus 384. 13. The SpectraMax iD3 reader is fully compatible with the SpectraDrop™0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The absorbance was measured at 620 nm using the microplate spectrophotometer (Spectramax 190, Molecular Devices Corporation, Sunnyvale, CA). Protein concentrations were calculated using. S. (volume is not critical because water does not absorb light appreciably between 190 and 900 nm) and read the plate. Introduction. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Description. The SpectraMax® ABS Plus is a compact UV-Vis absorbance microplate reader ideal for these types of quantitative assays. SpectraMax 190 Absorbance Microplate Readerwith laptop and softwareEnables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the. The light absorbance (A) of the supernatant was measured at 450, 532, and 600 nm using an MD SpectraMax 190 full-wavelength microplate reader . 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Modereader (SpectraMax 190, Molecular Devices, USA). Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. Streamline GLP/GMP compliance. The VersaMax and SpectraMax 190 read 96-well microplates. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and. . Standards were plotted using a log-log curve fit in SoftMax Pro Software. Condition:. The patented multichannel optical design of the SpectraMax 190 Absorbance. Spectramax® id3 multi-mode microplate reader, molecular devicesMolecular devices showcases spectramax I3x spectramax pdfSpectramax® i3:. Type: Clear: Sale. 9 to 190 ng/µL were prepared in TE buffer and pipetted onto the wells (spots) of the SpectraDrop Micro-Volume. ” We would like to show you a description here but the site won’t allow us. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. polynomial 2nd analysis from the protein standards. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Ultraviolet transparent multiwell plates from Greiner Highly recommended for measurements of DNA and protein concentrations at 260 nm or 280 nm, respectively By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The MIC was determined by the concentration where there was an obvious clear-cut decrease in the absorbance. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 0 out of 5 $ 4,499-5%. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. The SpectraMax Plus 384 reader is equipped with an innovation patented by Molecular Devices, the PathCheck® sensor, which measures the depth of a sample in a microplate by optical path length. Briefly, the. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. ; Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. A bandwidth of 5 nm or less is required for accurate absorbance measurement of DNA 1. The SpectraMax 190 Microplate Reader from Molecular Devices is ideal for UV-Vis life science applications, especially DNA analysis. Instrument pricing. . A SOFTMAX® PRO SOFTWARE Version 5 for Mac® and Windows® User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. The PathCheck sensor can extend the instrument linearity by a factor of three or more. Read - For the SpectraMax 190 and SpectraMax 340PC384, press to initiate the plate read when you operate the instrument in stand alone mode. 316 grating motor driver faulty. Multi-mode Microplate Readers. SoftMax® Pro Software User Guide — 0112-0140 Rev. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS control panel are shown in boxed Helvetica type. The temperature in the plate chamber is ambient and isothermal. Fluorescence Microplate Reader Comparison Chart. We don't know when or if this item will be back in stock. SpectraMax® 190. qPCR HBV DNA was extracted as. 118 Gemini XPS Fluorescence Microplate Reader . Seller InformationAnytimeLabTrader. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive measurementsofavarietyofanalytesacrossawiderangeofconcentrations. The same was done using samples of colloidal chitin to confirm concentrations. Spectranet customer portalGW9662 and roscovitine were purchased from MCE (catalog nos. . The M5 can read volumes down to 2uL with the uMax Low. The activity of catalase was expressed in μmol . The . 190-SC - SpectraMax 190 Microplate Reader with 1-Year Service Contract - SpectraMax 190 Absorbance Plate Reader, Molecular Devices - Each מותג: Molecular Devices 36,002. 2. com. Cell viability was denoted with relative growth rate (RGR%), which was calculated by using. These filter wheels are used for a host of applications, including color CCD photography, fluorescence microscopy, and photometry. Examples: fiPress carefully to engage,fl fiDo not press down. A wide linear dynamic range ensures accurate assay results across a broad span of sample types. absorbance readers. The SpectraMax 190 Microplate Reader is supplied with SoftMax Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software. Bioactivity assays of the complexes for calculating IC 50 were carried out with a SpectraMax 190 microplate reader (Molecular Devices, USA). Each sample has a discrete sample beam and reference. All samples were tested in duplicate and the optical density values were read on a microplate spectrophotometer (Spectramax 190, Molecular devices Corporation, CA, USA) at 450 nm. 96/384-well plate formats. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. 00 +$350. The optical density (OD) of the samples at 450 nm was measured by a SpectraMax 190, then the LDH activity was further calculated by a relevant formula. After incubation at 37 °C for 15 min, the resulting color reaction was read at 450 nm by a microplate reader (Spectramax 190, Molecular Devices Corporation, Sunnyvale, CA, USA) within 10 min, and the final IL-1 concentration was calculated using a 4-parameter logistic curve fit. Examples: “Press carefully to engage,” “ Do not press down. Molecular Devices SpectraMax 190 Microplate Reader. 0 nm full width half maximum. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. Microplate Readers Molecular Devices Spectramax iD3. 00. No accessories are needed and SoftMax® Pro. . The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Our absorbance plate readers feature our PathCheck Sensor technology and. 00 . Glutathione reductase (GR) activity was measured (at 25°C) according to the method of Carlberg and Mannervik . SPECTRAmax® 190. Since this is an older reader, we recommend that new users choose our newer model i3 reader. Learn more about Services for SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. Find regional support. Example: [Drawer]. T = I/I 0 and %T = 100 (T). The protein concentration was measured by an enhanced BCA protein assay kit (Beyotime; Nanjing, China). The SpectraTest validationIn this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. , Tokyo, Japan) was added to each well every 24 h. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method. 0 nm increments Wavelength bandwidth 2 nm Wavelength accuracy ±1. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. 0 nm, and a photometric accuracy of <±0. Molecular Devices also offers a mass spectrometry-based solution for food safety testingSpectraMax - 190 - Microplate Reader by Molecular Devices, LLC. e. 2) If you wish to regulate the temperature inside the microplate reading cham-. 2 nm * Wavelength Range 190 – 850 nm * Photometric. ®Molecular Devices SpectraMax 190 Microplate Reader. The SpectraMax ® Plus 384 Microplate Reader gives you walk-up convenience for both standard spectrophotometer and microplate reader applications on. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 305 reference level saturation. The reaction continued for 7 min and was subsequently stopped by adding 50 μL of 0. The ABS reader ranges from 340–850 nm in wavelength and the ABS Plus reader ranges from 190–1000 nm, which is equivalent to having 510 or 810 free filters, respectively. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. The . 6. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. The SpectraMax® 190 can read as low as 2μL with the μMax Low Volume Plate. Patient samples were diluted 1:100, except for patients with CML for whom a dilution of 1:300 was used. It may be named "Pothos". These robust readers have been placed in labs from Antarctica to the. Upgradeable modules are also available including western blot, cell imaging, and fast. Photometric Performance SpectraMax 190 Item Description. The negative control was cells in the fresh culture medium, and the positive control was cells in the culture medium that contained 0. Packed 25 plates per sleeve, 2 sleeves per case. SKU: 17247 Category: Laboratory Equip. SpectraMax® Paradigm® Multi-Mode Detection Platform. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. Molecular Devices Spectramax 190 Microplate Reader Molecular Devices Spectramax 190 Microplate Reader Specifications. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. 15 mmol). What is the part number for the filter wheel motor? I am looking to replace mine because I suspect it is the cause of the failure• SpectraMax® 190 (Serial#-NNR-xxxxx) •ersaMax™ (Serial#-BNR-xxxxx) V • AquaMax® Microplate Washer. The 3,3′,5,5′-tetramethylbenzidine (TMB) substrate was added after the incubation. The SpectraMax 190 reader is the tool for DNA analysis. Also refer to this Multimode Reader Comparison Chart. See More Information. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. SpectraMax® Paradigm® Multi-Mode Detection Platform. 306 plate air cal fail,low light. Molecular Devices SOFTmax PRO software is a highly integrated program that can be used to control and collect data from the SPECTRAmax 190. Wavelength range 190 nm to 850 nm. Stray light. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Check Details. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- The VersaMax and SpectraMax 190 read 96-well microplates. Analysis of pro-inflammatory macrophage phenotypic response to NAM and RSV. item 3 Molecular Devices SpectraMax 190 Microplate Spectrophotometer Molecular Devices SpectraMax 190 Microplate Spectrophotometer . it should turn freely, without any friction. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. . For very concentrated samples, use aSpectraMax iD3 reader. For very concentrated samples, use aBelow is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Not all manufacturers microplates are the same with regard to.